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Abstract
Evaluation of modified MRS media for the selective enumeration of Lactobacillus casei
Highlights
Lactobacillus is an extensive group of Gram-positive, catalase negative and non-spore forming organism belongs to Lactobacillaceae family
Significant white colonies with irregular edges of L. casei were observed on the modified MRS medium (Fig. 1) containing 1.5% calcium gluconate as compared to 0.5, 1.0 and 2.0% concentration/100ml of the medium while all the concentrations inhibited the growth of L. lactis which was unable to ferment calcium gluconate (Fig. 2)
On the basis of previous studies, MRS agar exhibits a low capability of discrimination among L. casei and L. lactis
Summary
Lactobacillus is an extensive group of Gram-positive, catalase negative and non-spore forming organism belongs to Lactobacillaceae family. L. casei is an obligate fermentative bacterium with complex nutritional requirements. It has its novel importance as a bio-preservative agent and enhancing food quality. Lactobacillus casei is Generally Recognized as Safe (GRAS). For the isolation and identification, cultural media is one of the most important tools and the methods to distinguish between Lactobacillus casei and Lactobacillus lactis are based upon various biochemical tests. MRS media is used for the isolation of Lactobacillus species but we have developed the modified selective medium that utilizes the calcium gluconate.
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