Evaluation of model parameters for growth, tannic acid utilization and tannase production in Bacillus gottheilii M2S2 using polyurethane foam blocks as support.

Abstract

Production of tannase from B. gottheilii M2S2 was studied under solid-state fermentation with an optimized medium consisting of polyurethane foam matrix of dimension 40×40×5mm, impregnated with a liquid medium comprising (w/v): 4% tannic acid; 2% NH4NO3; 0.1% KH2PO4; 0.2% MgSO4; 0.1% NaCl and 0.05% CaCl2·2H2O in distilled water, having a pH of 4.7. Maximum tannase production of 56.87 U/L was obtained after 32h of fermentation at 32°C in static condition. This study deals with the evaluation of unstructured kinetic models to understand the behavior of biomass, tannase production and tannic acid degradation, with the fermentation time. The growth rate of B. gottheilii M2S2 was 0.0703h-1 at 32h of fermentation. Product (Yx/s) and biomass yield (Yp/s) coefficients were estimated as 1.77 U/g of tannic acid and 0.276g of biomass/g of tannic acid. All the kinetic constants µ, α, β, m and n were evaluated using MATLAB 2015Rb program. The experimental and model-generated data showed a good correlation, which indicated that these models will describe tannase production and fermentation process.