Abstract
ABSTRACT: The somatic cell count (SCC) is a diagnostic tool that indicates the mammary gland health and can be determined by the counting of cells in the microscope. There are discussions regarding appropriate staining method to the ewes’ milk. The present study aimed to identify a methodology of microscopic SCC proper to the milk of the ovine species. Therefore, glass slides for smear were manufactured with 10 µL of ewe’s milk in 1 cm2, and the fixers xylol and Carnoy’s solution were tested, as well as and May-Grünwald, Broadhurst-Paley, Wrigth and Panoptic stainings. Carnoy’s solution was elected, because it allowed a better fixation of the dairy film to the microscope slides, and Broadhurst-Paley staining, due to its good coloration and visualization of cells, as well as the differentiation of cytoplasmic corpuscles in ewe’s milk. Broadhurst-Paley coloration is a tool applicable to the somatic cell count in ovine specie’s milk.
Highlights
The somatic cell count (SCC) is a diagnostic tool that indicates the mammary gland health and can be determined by the counting of cells in the microscope
The diagnosis of subclinical mastitis can be performed through the somatic cell count (SCC), i.e., the examination of structures of the organism defense before inflammation or infection and composed by immune system cells and epithelial cells (SUMMER et al, 2012)
As an indi‐ cator of the mammary gland health and the milk quality, the SCC is quite studied in bovine species (VIANA et al, 2010)
Summary
The somatic cell count (SCC) is a diagnostic tool that indicates the mammary gland health and can be determined by the counting of cells in the microscope. The fixed slides were subjected to one of the four staining procedures: May‐Grünwald‐Giemsa (Laborclin Produtos para Laboratórios, Pinhais, Paraná, Brazil); Wright (Laborclin Produtos para Laboratórios, Pinhais, Paraná, Brazil); and Panótico (Laborclin Produtos para Laboratórios, Pinhais, Paraná, Brazil), according to the manufacturer’s instructions, and Broadhurst‐Paley as described by SCHALM et al (1971).
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