Evaluation of MicroRNA-210(miR-210) as a Diagnostic and Prognostic Biomarker in Pre-eclampsia Pregnancies

Abstract

Objective: This study aims to assess the level of miR-210 in Egyptian women with pre-eclampsia (PE) and to evaluate its role in diagnosis and prognosis of the disease. Subjects & Methods: The study was conducted on 30 pregnant women with PE divided into two groups: group (I): included 15 cases with mild PE and group (II): included 15 cases of severe PE and, 20 healthy pregnant women with matched age and sex were included as control group. All women included in the study were subjected to, history taking full clinical examination, laboratory investigations included (CBC), (PT), (ALT, AST), (urea, creatinine), detection of protein in urine, as well as miRNA-210 gene expression by RT-PCR. Results: Patients with PE showed a highly significantly increase in serum miR-210 (P value <0.001) compared to control as well as, it was higher in severe PE than in mild PE (P value <0.001). MiR 210 have highly significant positive correlation with (systolic, diastolic, MABP), (Proteinuria) and (PT) a significant positive correlation with (AST), (ALT),.However no significant correlation was found with (Hb%) ,platelet count, PTT and INR, serum urea and creatinine. PTT was statistically significant (P value 0.002).The best cutoff value of PE (2.03) with sensitivity and specificity of 90.0% and 85.0% respectively. Conclusion: Expression of mir-210 is upregulated in pre-eclampsia and was higher in severe than in mild. Hence, the serum miR-210 can be used as a diagnostic, prognostic biomarker in PE patients and understanding pathophysiology.