Abstract
Nodal explants of Bacopa monnieri were cultured in a solid Murashige and Skoog (MS) medium containing 1 mg L−1 kinetin, transferred after 30 days to a liquid MS medium of the same composition in 100 mL and 250 mL Erlenmeyer flasks (with 10 mL and 50 mL medium, respectively) and Phyta Jars (with 10 mL medium) and subcultured every 7 d. Shoot doubling time was estimated and the total antioxidant capacity of aqueous extracts from liquid-cultured and acclimatized plants was studied. In the solid medium, 5 rooted shoots/explant were produced after ~20 days, with shoot doubling time of 7.99 days; in liquid media, 36–75 rooted shoots were produced with shoot doubling time of 0.91–1.17 days. Aqueous extracts of acclimatized plants showed 10-fold higher total antioxidant capacity compared to in vitro liquid cultured plantlets.
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