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Abstract
The capture of circulating tumor cells (CTCs) is still a challenging application for microfluidic chips, as these cells are rare and hidden in a huge background of blood cells. Here, different microfluidic ceiling designs in regard to their capture efficiency for CTCs in model experiments and more realistic conditions of blood samples spiked with a clinically relevant amount of tumor cells are evaluated. An optimized design for the capture platform that allows highly efficient recovery of CTCs from size-based pre-enriched samples under realistic conditions is obtained. Furthermore, the viability of captured tumor cells as well as single cell recovery for downstream genomic analysis is demonstrated. Additionally, the authors' findings underline the importance of evaluating rational design rules for microfluidic devices based on theoretical models by application-specific experiments.
Highlights
Circulating tumor cells (CTCs) in patient blood is associated with for microfluidic chips, as these cells are rare and hidden in a huge background of blood cells
In order to assess the influence of the different staggered herringbone (SHB) structures in the chip ceiling (Figure 2) on the capture efficiency of the chip platform, three different designs were produced (Table 1, called ceiling type HA, HB, HC) and compared with each other as well as an unstructured microfluidic channel
We investigated the influence of ceiling structure and target cell concentration on the capture efficiency of
Summary
In order to assess the influence of the different SHB structures in the chip ceiling (Figure 2) on the capture efficiency of the chip platform, three different designs were produced (Table 1, called ceiling type HA, HB, HC) and compared with each other as well as an unstructured microfluidic channel. The herringbone is composed of a series of chevrons with a long and a short groove. The short groove covers one third of the symmetry width whb and the long groove covers the remaining two thirds. The angle between grooves θhb is 90° and the angle with the channel wall θc is 45°. Each chevron is whb wide and placed side by side to cover the complete channel width wc. Www.adv-biosys.com the SHB design that was utilized in a previous publication by our group, labeled HA, and a smooth channel with height hc = 150 μm were included in the comparison. All designs have the following parameters in common: complete channel width wc = 9600 μm, number of grooves per partcycle Ng = 5, angle of herringbone θhb = 90° and the angle with channel wall θc = 45°
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