Evaluation of microbial colony growth parameters by laser speckle imaging

Abstract

Our previous studies have shown that it is possible to determine the growth activity of a microbial colony by laser speckle imaging techniques. A sub-pixel correlation method was proposed to detect small changes in the sequence of laser speckle images. In this study, we compared the laser speckle imaging method to the reference method - image time series under white light to detect the colony growth parameters (growth rate, critical detection time).