EVALUATION OF METABOLISM PARAMETRES OF CONNECTIVE TISSUE IN TREATMENT OF EXPERIMENTAL PURULENT WOUNDS OF CATS

Abstract

Wound pathology is one of the most important problems of modern veterinary surgery. Violation of connective tissue metabolism significantly affects occurrence and development of many pathological processes in various organs and tissues, however, parametres of connective tissue metabolism in purulent wounds of cats have not been thoroughly studied. Therefore, their study will allow to substantiate pathogenetically and improve treatment methods of purulent wounds of cats. The studies were carried out from 2010 to 2020 on the basis of the clinic of veterinary medicine and the laboratory of the Department of Surgery and Diseases of Small Animals of Luhansk State Agrarian University, as well as in biochemical and immunological laboratory of Lugansk. Twelve animals were used in the experiment, which were divided into two groups (the first - experimental, the second - control). The ointment "Methyluracil with Miramistin" (Darnitsa) with addition of 1% hyaluronic acid and 1% trifusol were used in the experimental group. Only "Methyluracil with Miramistin" ointment was used for treatment of animals in the control group. Animals underwent daily revision of wounds and local mechanical treatment. For planimetric studies, a ruler and a transparent film with a grid on it with a cell size of 1 cm2 were used. As for animals of the experimental group, content increase of hexoses associated with proteins, hexoses of glycoproteins and sialic acids was recorded only during the period of inflammatory and degenerative changes. Subsequently, starting from the 12th day of the experiment, these parametres decreased to the initial level. As for animals of the control group, the content of hexoses associated with proteins gradually decreased, but was higher than that of cats of the experimental group due to increase of hexoses of glycosaminoglycans. Also, the content of sialic acids in the control group remained high throughout the experiment. Such tendency indicates that application of ointment with addition of hyaluronic acid and trifusol contributes to normalization of the H-GAG / H-GP index, which is typical for a more intensive development of reparative processes. On the contrary, an increase of the healing time of a wound defect of animals of the control group was accompanied by a redistribution of hexose fractions towards an increase in the proportion of glycosaminoglycans. These changes are stipulated by gradual depolymerization and restructuring of the basic substance of the connective tissue in the phase of regeneration and proliferation of the wound process.