Evaluation of mechanical transmission of Enterococcus spp., resistant to antibiotics, having flies as vectors of dissemination

Abstract

The objective of this work was to evaluate the mechanical transmission of Enterococcus spp., resistant to antibiotics, using flies as dissemination vectors. In this work, 50 flies were captured, 25 in “La Llanada” community, in Cumaná and 25 others in the surroundings of the “Juan Otaola Rogliani” outpatient clinic and the central cemetery of Carúpano. The main families of flies captured in the state of Sucre were Muscidae, Calliphoridae and Sarcophagidae. The flies captured in Cumaná had fewer strains of Enterococcus spp., on the surface than those from Carúpano, while those from Cumaná were more colonized in the intestine than those from Carúpano. A total of 14 strains of Enterococcus were isolated, among which were E. faecium (6%), E. gallinarum (36%) and E. casseliflavus (58%). The susceptibility profile of E. casseliflavus strains is resistance to linezolid, tetracycline, erythromycin, rifampicin and intermediate susceptibility to fluoroquinolones; E. gallinarum strains were sensitive to rifampicin and tetracycline, and resistant to the other antibiotics. The only strain of E. faecium isolated in Carúpano, presented low level of resistance to vancomycin. It did not amplify for the glycopeptide resistance ligase genes vanA, vanB, vanD, vanE, or vanG. Antibiotypes IA and IV of E. caseliflavus were detected in both Carúpano and Cumaná.