Abstract
Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories. To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification. The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms. MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level. MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology. State Board of Medical Research (SBMR).
Highlights
Microbial identification in laboratories is achieved mainly by observing colony morphology, grams stain and biochemical reactions.[1]
For MALDI-TOF MS, Isolate was smeared on the target slide with a wooden stick and was smeared with 1 μL VITEK MS-cyano‐4‐hydroxycinnamic acid (CHCA) (α-Cyano-4-hydroxycinnamic acid) and air-dried until the matrix and sample co-crystallized
The identification rates for MALDI- TOF MS and VITEK-2 up to species level were 92.9% (92/99) and 90.9% (90/99) respectively (One Minor error was not taken for calculation up to species level as Sequencing was not done for this isolate)
Summary
Microbial identification in laboratories is achieved mainly by observing colony morphology, grams stain and biochemical reactions.[1]. VITEK 2 can accurately identify 84.7% of the gram-negative bacteria within three hrs as shown by Funke et al .4 MALDI-TOF MS technology has a simple, high throughput, and low-cost technique plus a larger database and a more rapid turnaround time (few minutes) .5 The prime objective was to use two systems to identify 100 isolates
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