Abstract
In this research, extraction of bovine serum albumin (BSA) with aqueous two-phase systems (ATPS) based on polyethylene glycol/sodium citrate using the microfluidic apparatus were investigated. The operational variables were studied using microchannel width (800–400 μm), salt phase flow rate (1.6–4 mL/h), polyethylene glycol-rich phase flow rate (0.8–2 mL/h), and pH (5–9) by response surface methodology (RSM). Double interfaces performed BSA transfer from the salt phase to the polyethylene glycol (PEG) phase. The effects of adding the interface on the mass transfer coefficient and the mass transfer rate were evaluated. Using the microfluidic system due to having two interfaces will increase the extraction rate compared to macro systems with a single interface. In values, salt phase pH of 7, PEG phase flow rate of 2 mL/h, and salt phase flow rate of 4 mL/h were the optimum condition for BSA extraction rate (70.9%.) in this study.
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