Abstract
Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g.
Highlights
In order to analyze the processes of modification and immobilization, infrared spectroscopy (FTIR) was performed, using the PLATINUM-ATR infrared spectrometer (Bruker)
For the synthesis of maghemite, was used 250oC instead of 200oC to avoid the inversion of the maghemite structure in hematite, considering its minimum inversion temperature (Tinv) at 250oC (DUNLOP and ÖZDEMIR, 1997)
During the synthesis of maghemite, the change in color of the solution from ferric chloride to dark red after the addition of sodium sulfite solution is justified by the formation of the complex [Fe2(SO3)]4+, making the solution yellow with the total reduction of dissolved iron
Summary
O. DOMINGUES1,*, L. K. SANTOS2, R. D. HERCULANO1, D. L. FLUMIGNAN2,3 and A. V. PAULA1 Department of Engineering Bioprocesses and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil. Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil. Campus Cuiabá, Mato Grosso Federal Institute of Education, Science and Technology (IFMT), Cuiabá, Mato Grosso, Brazil.
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