Abstract
<p class="Abstract">The present study was commenced to evaluate the anti-arthritic effect of 70% methanol extract and <em>n</em>-butanol and aqueous fractions of <em>Berberis calliobotrys</em> using both <em>in vitro</em> and <em>in vivo</em> arthritis models. Extract and fractions were investigated<em> in vitro</em> for inhibition of protein (bovine serum and egg albumin) denaturation and human red blood cell membrane stabilization. <em>In vivo</em> anti-arthritic activity of extract and fractions at 50, 100 and 200 mg/kg was assessed using turpentine oil and formaldehyde-induced arthritis, while, 200 mg/kg dose was evaluated against complete Freund’s adjuvant-induced arthritis. <em>B. calliobotrys</em> produced significant (p&lt;0.001) dose dependent inhibition of protein denaturation and human red blood cell membrane stabilization. In turpentine oil, formaldehyde and complete Freund’s adjuvant-induced arthritis models,<em> B. calliobotrys</em> significantly (p&lt;0.001) reduced joint and paw swelling. <em>B. calliobotrys</em> markedly improved body weight, hematology profile, radiological and histopathological parameters in complete Freund’s adjuvant model. It could be concluded that <em>B. calliobotrys</em> holds anti-arthritic potential, supporting its traditional use in treatment of rheumatoid arthritis.</p><br /><p> </p>
Highlights
Rheumatoid arthritis is a chronic inflammatory, autoimmune disorder that influence multiple joints (Afuwape et al, 2002)
The present investigation was carried out to scrutinize the anti-arthritic effect of traditionally used plant B. calliobotrys
In current investigation aqueous methanol extract of B. calliobotrys showed maximum inhibition of protein denaturation, whereas, among fractions butanolic fraction has been found to produce comparable antidenaturation effect with standard drug (Table I). It can be assumed on the basis of present findings that the ability of preventing protein denaturation of B. calliobotrys could be responsible for its antiarthritic effect
Summary
Rheumatoid arthritis is a chronic inflammatory, autoimmune disorder that influence multiple joints (Afuwape et al, 2002). This disease is accompanied by synovial inflammation and hyperplasia, autoantibody production, cartilage and bone deformation with functional impairment and systemic complications e.g., cardiovascular, pulmonary, psychological, and skeletal disorders (McInnes and Schett, 2011). Therapy includes disease modifying anti-rheumatic drugs, nonsteroidal anti-inflammatory drugs, biologic response modifiers and corticosteroids. These drugs are currently being used but their toxic profile prevail over beneficial effects, decreasing patient adherence to therapy. Berberis calliobotrys Bienert ex Aitch, commonly known as Chowenj and Zarch, is a medicinally imperative plant having folkloric use in arthritis by aboriginal people
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