Abstract
For analyzing detailed and localized biological phenomenon, a local environmental control technique is important. In here, we developed a local environmental chemical stimulation system. By using micro pipettes, this system could control the local reagent concentration dynamically, freely and automatically. In this system, to reduce the diffusion of chemical solution, spout pipette and suction pipette were used. Furthermore, as an evaluation of this system, peeling off of certain area of confluent cells was demonstrated.
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