Abstract
Breast cancer is one of the common malignant tumors, and liquid biopsy has become a hot spot for clinical testing. To clarify the detection effect of liquid biopsy in breast cancer, we collected peripheral blood of HER2-positive (human epidermal growth factor receptor 2-positive) patients. Circulating tumor cells (CTCs) were isolated and analyzed. HER2 expression on CTCs was detected. The results showed that in the 198 HER2-positive samples, the CTC detection rate was 79.8% (158/198), and the mean number of CTCs was 21, ranging from 1 to 63/7.5 mL peripheral blood. Only 41.1% (65/158) of patients had histology and CTC HER2 status consistent with the remaining 58.9% (93/158) of patients, although their histological HER2 was positive, and CTC HER2 was negative. Our study confirmed the value of CTC HER2 real-time status testing in HER2-positive breast cancer patients. The inconsistency in HER2 status between CTCs and histology may be related to the time interval between CTCs and histological HER2 detection, suggesting that real-time HER2 detection is necessary for histological HER2-positive patients.
Highlights
Breast cancer is one of the most common female tumors, and GLOBOCAN predicts that there will be 210,000 new cases of breast cancer in China in 2020 [1]
In many markers related to breast cancer, estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), and Ki-67 are considered to be the markers for breast cancer [2, 3]
The results of immunohistochemistry and immunofluorescence showed that the HER2 protein was mainly expressed in the cell membrane of breast cancer, which was brown (Figures 1(a) and 1(b))
Summary
Breast cancer is one of the most common female tumors, and GLOBOCAN predicts that there will be 210,000 new cases of breast cancer in China in 2020 [1]. The occurrence and development of breast cancer is regulated by multiple genes. HER2 is a very important molecular marker for breast cancer. About 20% of newly diagnosed breast cancer patients are HER2-positive [4, 5]. The main difficulty in tumor treatment is that the cause is complex and constantly changing, and the etiology is complex and constantly changing, and the status of the drug target is not 100% predictive of efficacy [6, 7]. For more accurate and effective treatment, it is necessary to evaluate the efficacy in a timely manner and adjust the treatment strategy in a timely manner. Traditional evaluation methods do not meet the above requirements [8,9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
