Evaluation of laccase production by two white-rot fungi using solid-state fermentation with different agricultural and forestry residues

Abstract

Pleurotus ostreatus and a newly isolated Ganoderma lingzhi strain were evaluated for their laccase secretion capacity by solid-state fermentation with different agricultural and forestry residues. There was a significant difference among fungi for biosynthetic potential. In principle, the laccase secretion capacity of P. ostreatus CY 568 was stronger than that from G. lingzhi Han 500. Different species of fungi had a preference for agricultural and forestry residues. The presence of cottonseed hull and Populus beijingensis were helpful for accelerating the rate of laccase enzyme production of P. ostreatus CY 568. Cottonseed hull and corncob were useful for improving the production of laccase from G. lingzhi Han 500. Continuous and stable laccase production was found on cottonseed hull by P. ostreatus CY 568 and G. lingzhi Han 500. Maximum laccase activity obtained from P. ostreatus CY 568 on Toona sinensis, Sophora japonica, Salix babylonica, Populus beijingensis, corncob, cottonseed hull, and straw of Oryza sativa was higher than that from G. lingzhi Han 500, and was nearly 1.16-fold, 1.59-fold, 3.32-fold, 1.39-fold, 1.08-fold, 1.08-fold, and 1.36-fold, respectively. These findings will be helpful for developing new productive strains and expanding more species for industrial application to obtain efficient and low-cost laccase.