Evaluation of key microbial community succession and enzyme activities of nitrogen transformation in pig manure composting process through multi angle analysis

Abstract

This experiment aimed to investigate changes in enzyme activity, microbial succession, and nitrogen conversion caused by different initial carbon-to-nitrogen ratios of 25:1, 35:1 and 20:1 (namely CK, T1 and T2) during pig manure composting. The results showed that the lower carbon-to-nitrogen ratio (T2) after composting retained 19.64 g/kg of TN which was more than 16.74 and 17.32 g/kg in treatments of CK and T1, respectively, but excessive conversion of ammonium nitrogen to ammonia gas resulted in nitrogen loss. Additional straw in T1 could play the role as a bulking agent. After composting, TN in T1 retained the most, and TN contents were 63.51 %, 67.34 % and 56.24 % in CK, T1 and T2, respectively. Network analysis indicated that many types of microorganisms functioned as a whole community at various stages of nitrogen cycle. This study suggests that microbial community structure modification might be a good strategy to reduce ammonium nitrogen loss.