Evaluation of Juvenile Freshwater Mussel Sensitivity to Multiple Forms of Florpyrauxifen-Benzyl.

Abstract

ProcellaCOR® (active ingredient [ai], florpyrauxifen-benzyl) is an aquatic herbicide registered for use in 2018 for managing invasive and nuisance macrophyte species. Registration studies evaluating its acute toxicity revealed a favorable environmental profile; however, prior to this study, no information existed on the toxicity of florpyrauxifen-benzyl to native freshwater mussels (Family Unionidae), one of the most sensitive and imperiled faunal groups globally. We followed standard acute (96h) toxicity test guidelines and exposed juvenile Fatmucket (Lampsilis siliquoidea) and Eastern Lampmussel (Lampsilis radiata) to the following formulations or compounds: ProcellaCOR SC and EC formulations, technical grade active ingredient (TGAI, florpyrauxifen-benzyl), and an analytical-grade sample of the weaker florpyrauxifen acid (FA). In all tests, the estimated median lethal concentrations to produce 50% mortality (LC50) were greater than the highest concentration tested of each formulation or compound. The no observable adverse effect concentrations (NOAEC, based on analytical recoveries measured at the highest concentration tested where no toxicity was observed) were TGAI = 26µg/L, FA = 100,000µg/L, ProcellaCOR® SC = 193µg ai/L ProcellaCOR® EC = 585µg ai/L and the NOAEC values for the registered commercial formulation products (ProcellaCOR® SC and ProcellaCOR® EC) were orders of magnitude greater (3.9× and 11.7×, respectively) than the maximum application rate (50µg/L). Our results show that the herbicide formulations and compounds tested were not acutely toxic to juveniles of these two species of freshwater mussels, indicating minimal risk of short-term exposure from florpyrauxifen-benzyl applications in the environment for aquatic weed control. However, potential chronic or sublethal effects remain uncharacterized and warrant additional investigation.