Evaluation of iron transport from ferrous glycinate liposomes using Caco-2 cell model.

Ding Baomiao,Li Li,Yi Xiangzhou,Yang Hualin

doi:10.4314/ahs.v17i3.37

Abstract

Iron fortification of foods is currently a strategy employed to fight iron deficiency in countries. Liposomes were assumed to be a potential carrier of iron supplements. The objective of this study was to investigate the iron transport from ferrous glycinate liposomes, and to estimate the effects of liposomal carriers, phytic acid, zinc and particle size on iron transport using Caco-2 cell models. Caco-2 cells were cultured and seeded in DMEM medium. Minimum essential medium was added to the basolateral side. Iron liposome suspensions were added to the apical side of the transwell. The iron transport from ferrous glycinate liposomes was significantly higher than that from ferrous glycinate. In the presence of phytic acid or zinc ion, iron transport from ferrous glycinate liposomes and ferrous glycinate was evidently inhibited, and iron transport decreased with increasing phytic acid concentration. Iron transport was decreased with increase of particle size increasing of ferrous glycinate liposome. Liposomes could behave as more than a simple carrier, and iron transport from liposomes could be implemented via a mechanism different from the regulated non-heme iron pathway.

Highlights

Iron fortification of foods is currently a strategy employed to fight iron deficiency in countries
Iron transport of ferrous glycinate liposomes was in agreement with that of ferrous glycinate, which was time-dependent for the period of 120 min, and the iron tended to increase with increasing incubation time
The relative iron transport of ferrous glycinate liposomes at 1, 10, and 50 μmol/L and incubation for 120 min were 146.1%, 131.1%, and 128.9%, respectively, which indicated that the iron of ferrous glycinate liposomes across Caco-2 cells could be more efficient

Summary

Introduction

Iron fortification of foods is currently a strategy employed to fight iron deficiency in countries. Objective: The objective of this study was to investigate the iron transport from ferrous glycinate liposomes, and to estimate the effects of liposomal carriers, phytic acid, zinc and particle size on iron transport using Caco-2 cell models. Results: The iron transport from ferrous glycinate liposomes was significantly higher than that from ferrous glycinate. In the presence of phytic acid or zinc ion, iron transport from ferrous glycinate liposomes and ferrous glycinate was inhibited, and iron transport decreased with increasing phytic acid concentration. Iron transport was decreased with increase of particle size increasing of ferrous glycinate liposome. Evaluation of iron transport from ferrous glycinate liposomes using Caco-2 cell model.

Objectives

Methods

Results