Evaluation of insulin sensitivity by hyperinsulinemic–euglycemic clamp technique in Chinese patients with type 1 diabetes mellitus

Abstract

Objective To investigate insulin sensitivity in Chinese adult patients with type 1 diabetes mellitus through hyperinsulinemic–euglycemic clamp technique. Methods Adult patients with type 1 diabetes mellitus (n=10, group T1DM) and age, sex, body mass index, waist and waist-hip ratio matched normal glucose tolerance subjects (n=10, group NGT) were recruited from the third affiliated hospital of Sun Yat–sen University between June 2011 and June 2013. There were 5 male and 5 female in each group. The average age was (27±5) and (27±8) yrs in each group, respectively. Hyperinsulinemic-euglycemic clamp were performed to measure glucose disposal rate as insulin sensitivity. Differences between groups were analyzed by Student's t test. Results No significant differences were found in age, waist, waist-hip ratio, body mass index, systolic blood pressure, diastolic blood pressure, triglyceride, low – density lipoprotein–cholesterol, high–density lipoprotein–cholesterol and uric acid between type 1 diabetes mellitus group and normal glucose tolerance group (t=- 1.248- 0.973, all P>0.05), except for glycated hemoglobin A1c ((7.5± 0.8)% vs (5.2± 0.3)%,t=- 6.318, P<0.001). Glucose disposal rate in type 1 diabetes mellitus and normal glucose tolerance groups were (7.8±1.9) mg·kg-1·min–1 and (14.0±1.7) mg·kg–1·min–1 (t=7.769, P<0.001), respectively. Conclusion Insulin resistance may be present in Chinese patients with adult type 1 diabetes mellitus. Key words: Diabetes mellitus, type 1; Insulin resistance; Hyperinsulinemic–euglycemic clamp technique