Abstract
Tyrosinase or polyphenol oxidase (EC 1.14.18.1) is a copper-containing enzyme with widespread occurrence in living organisms including microorganisms, plants and animals.This enzyme catalyses a two-step reaction, the hydroxylation of a monophenol and the conversion of an o-diphenol to an o-quinone. From pathological and economical point of view, tyrosinase is very important and interesting enzyme which attracted the attention of researchers.Due to the key role of tyrosinase in melanogenesis and fruit browning, several inhibitors from both natural and synthetic sources have been identified.In this study we attempted to evaluate the inhibitory effects of some natural compounds from plant sources on mushroom tyrosinase activity. Experimentally, traditional spectroscopic methods of tyrosinase assay were applied and the mechanism of inhibition and kinetic parameters are determined. Computationally, the binding capability of the proposed natural products was evaluated by docking methods.
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