Evaluation of inhibition of F4ac positive Escherichia coli attachment with xanthine dehydrogenase, butyrophilin, lactadherin and fatty acid binding protein.

Predrag Novakovic,Betty Lockerbie,Matthew E Loewen,Elemir Simko,Chandrashekhar Charavaryamath,Igor Moshynskyy,Chris Stuart,Beverly A Kidney,Radhey S Kaushik

doi:10.1186/s12917-015-0528-0

Abstract

BackgroundNeonatal and post-weaning colibacillosis caused by enterotoxigenic E. coli is responsible for substantial economic losses encountered by the pork industry. Intestinal colonization of young piglets by E. coli depends on the efficiency of bacterial attachment to host gastrointestinal epithelium that is mediated by fimbriae. We tested the effect of porcine individual milk fat globule membrane (MFGM) proteins on F4ac positive E. coli attachment to porcine enterocytes in vitro.ResultsButyrophilin, lactadherin and fatty acid binding protein inhibited fimbriae-dependent adherence of E. coli to enterocytes in vitro, while xanthine dehydrogenase did not. The inhibiting activity was dose-dependent for all three proteins, but the inhibiting efficiency was different.ConclusionsThe results indicate that MFGM proteins may interfere with attachment of E. coli to porcine neonatal intestinal mucosa.

Highlights

Neonatal and post-weaning colibacillosis caused by enterotoxigenic E. coli is responsible for substantial economic losses encountered by the pork industry
We identified the individual proteins of porcine milk fat globule membrane (MFGM) that have binding affinity for F4ac fimbriae of enterotoxigenic Escherichia coli (ETEC) [4,5] and demonstrated that porcine lactadherin interfered with attachment of F4+ ETEC to intestinal villi in vitro [4]
IPEC-J2 cell line and culture conditions The IPEC-J2 [undifferentiated porcine intestinal epithelial cell line derived from porcine jejunum, a kind gift from Dr Pradip Maitii (Nutratechglobal, Winnipeg, MB, Canada)] cells were seeded on cell culture flask (T75cm2, Corning, NY, USA) as described previously [6]

Summary

Introduction

Neonatal and post-weaning colibacillosis caused by enterotoxigenic E. coli is responsible for substantial economic losses encountered by the pork industry. Post-weaning diarrhea (PWD) due to F4ac-positive enterotoxigenic Escherichia coli (ETEC) is an important cause of morbidity and mortality in weaned piglets [1]. PWD is controlled by various management strategies, use of antibiotics as feed supplements and/or immunization with vaccines containing fimbrial antigens. None of these control measures can completely eliminate PWD from modern swine production. Continuous use of sub-therapeutic doses of antibiotics as feed supplements potentially leads to the emergence of genes encoding antimicrobial resistance in porcine microflora. These antimicrobial resistant genes may be incorporated by animal and human pathogens, potentially causing serious public health problems. There is a great demand to Novakovic et al BMC Veterinary Research (2015) 11:238

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