Evaluation of inflammatory miRNA155 and 146a expression in heart tissue of ovalbumin-sensitized male rats

Mehdi Hassanpour,Rana Keyhanmanesh,Reza Rahbarghazi,Akbar Darbin,Mahdi Ahmadi

doi:10.34172/jrcm.2021.011

Abstract

Introduction: Asthma is a chronic pulmonary inflammation occurred in response to different allergens, leading to respiratory system insufficiency. The production of different inflammatory factors and enhanced immune system response may affect the function of other organs. The aim of this study was to investigate the expression of inflammatory microRNAs in cardiac tissue in asthmatic rat model. Methods: In this study, the animals were allocated into Control and Asthmatic rats (n=8). To induce asthma, rats were challenged with ovalbumin. 14 days after induction of asthma, rats were euthanized and Hematoxylin-Eosin staining was performed to assess pathological changes in pulmonary tissue. Serum levels of cardiac enzymes were measured using ELISA kits. Finally, transcription level of inflammatory miRNAs, miRNA-146a and -155, were measured using real-time PCR analysis. Results: Based on our findings, histological examination indicated the existence of pathological changes in pulmonary tissue after asthma induction. Bright-field analysis revealed an existence of inflammatory response and cytotoxicity in cardiac tissue. Also, the serum levels of CpK-MB, ALT, and AST were significantly higher in the serum of asthmatic group compared to control group (p<0.05). Finally, asthmatic condition induced the expression of (2-fold) miRNA-146a and (1.5-fold)-155 in cardiac tissue, respectively. Conclusion: As a conclusion, it could be concluded that asthmatic condition induces systemic inflammation in cardiac tissue. On a more general note, we propose that therapeutical approaches directed to inflammatory pathway may be required to preserve cardiac injuries caused of asthma.

Highlights

Asthma is a chronic pulmonary inflammation occurred in response to different allergens, leading to respiratory system insufficiency
Asthma promoted pathological changes in pulmonary and cardiac tissues Histological examination revealed the promotion of pathological changes in pulmonary tissue after induction of asthma
More than a decade, studies have suggested that different dysfunctional conditions, such as asthma, lead to cardiovascular disease (CVD).[19,20,21]

Summary

Introduction

Asthma is a chronic pulmonary inflammation occurred in response to different allergens, leading to respiratory system insufficiency. Asthma is a chronic pulmonary inflammatory disease with profound structural alteration in lungs and airway conduits, contributing to airway obstruction.[1,2] The emergence of chronic inflammatory response, , could affect the function of respiratory system, and affect the physiological behavior of tissues which functionally are in close relation with respiration.[3] Evidences have shown the potency of asthma inflammatory factors in the progression of atherosclerosis and cardiovascular disease (CVD).[4] Based on the released facts from cohort studies, there is a close association between asthma and CVDs, in which an increased risk of CVDs has been reported in asthmatic patients.[5] there is no consolidated agreement on the reciprocal association of CVD and asthmatic changes and underlying mechanisms are still unknown.[6] It is thought that the increase of acute phase proteins and inflammatory mediators in asthmatic patients such as including interleukin-1 (IL-1), tumor necrosis factoralpha (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), platelet-activating factor, etc., can play a role in the progression of atherosclerosis and CVD.[7] Along with these changes, the level of leukotriene is increased after initiation of inflammation in cardiac and pulmonary tissues.[8]

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