Evaluation of Infectivity and Immunogenicity of Sugar Stabilized Nairobi Sheep Disease Vaccine

Abstract

This work presents a cost-effective approach that can tremendously reduce the financial and logistical burden for vaccine delivery. Strict requirement of a cold chain account about 80% of the total cost of vaccination program. This research paper detail the use of inexpensive FDA approved biocompatible sugars for stabilizing inactivated Nairobi sheep disease virus vaccine. We evaluated sugar additives that improve the storage and maintain the antigenic properties of inactivated vaccine. Low concentrations of trehalose and sucrose maintain the residual infectivity of the virus. Nairobi sheep disease virus vaccine was lyophilized in trehalose and sucrose and subjected to accelerated stability test at temperatures; 25 0C, 37 0C and 40 0C. Results of this study showed the sugar stabilizers can retain residual infectivity for 14 days at 400C. Stabilization effect of trehalose and sucrose preserve the efficacy of inactivated vaccine stored at 40 0C for despite loss of infectivity.