Abstract
To develop a suitable Saccharomyces cerevisiae industrial strain as a chassis cell for ethanol production using lignocellulosic materials, 32 wild-type strains were evaluated for their glucose fermenting ability, their tolerance to the stresses they might encounter in lignocellulosic hydrolysate fermentation and their genetic background for pentose metabolism. The strain BSIF, isolated from tropical fruit in Thailand, was selected out of the distinctly different strains studied for its promising characteristics. The maximal specific growth rate of BSIF was as high as 0.65 h−1 in yeast extract peptone dextrose medium, and the ethanol yield was 0.45 g g−1 consumed glucose. Furthermore, compared with other strains, this strain exhibited superior tolerance to high temperature, hyperosmotic stress and oxidative stress; better growth performance in lignocellulosic hydrolysate; and better xylose utilization capacity when an initial xylose metabolic pathway was introduced. All of these results indicate that this strain is an excellent chassis strain for lignocellulosic ethanol production.
Highlights
Biofuels, chemicals and other commodities produced from renewable and abundant lignocellulosic feedstocks have become increasingly important because of the depletion of fossil fuel energy sources and growing public concerns about the environment and food security (Zhou et al, 2012; Jönsson et al, 2013)
A high glucose metabolism capacity and ethanol yield, and the capacity to tackle the challenges associated with lignocellulose fermentation are necessary properties for a lignocellulosic ethanol-producing strain
Fermentation performance of S. cerevisiae strains on glucose
Summary
Biofuels (including ethanol, butanol and biodiesel), chemicals and other commodities produced from renewable and abundant lignocellulosic feedstocks have become increasingly important because of the depletion of fossil fuel energy sources and growing public concerns about the environment and food security (Zhou et al, 2012; Jönsson et al, 2013). One of the most practical solutions is to produce bioethanol from lignocellulosic feedstocks with Saccharomyces cerevisiae (Palmqvist and Hahn-Hägerdal, 2000a). This natural ethanol producer faces several new challenges when the substrate is lignocellulose instead of starch. A high glucose metabolism capacity and ethanol yield, and the capacity to tackle the challenges associated with lignocellulose fermentation are necessary properties for a lignocellulosic ethanol-producing strain. The lignocellulosic ethanol conversion process generally includes raw material pretreatment, cellulose hydrolysis, sugar fermentation by microorganisms and distillation. The yeast strain used in lignocellulosic bioethanol production requires high ethanol yields from both glucose and xylose, and robustness in its harsh working environment
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
