Abstract
A comparative study of the retention behaviour of disubstituted benzene derivatives on (2-hydroxy-3-methacryloyloxypropyl β-cyclodextrin-co-N-vinylpyrrolidone) copolymers physically adsorbed onto porous silca gel was achieved. Under reversed-phase mode, the separation process is based on an inclusion complex formation, and the inclusion selectivity is strongly influenced by hydrogen bonds and/or steric effects. The influence of cyclodextrin derivatives used as mobile phase additives for the conventional reversed-phase high-performance liquid chromatography was also examined with the aim to confirm the formation of inclusion complexes between CD derivatives and test molecules. Under normal-phase mode, it was found that the retention and separation processes are mainly controlled by hydrophilic interactions between the polar substituents of the solute and the hydroxyl groups of the cyclodextrin units. Finally, the enantiomer separation ability was briefly demonstrated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
