Evaluation of in vivo wound healing activity of Plumeria obtusa L. (Champa) spray in rats

Abstract

Wound healing is a general repair response or process of the body immediately after the disruption of the skin integrity. However wound healing is a complex process in which body repairs itself. The aim of this research is to study the wound healing activity of ethanolic extract of Plumeria obtusa L. leaves and to study the efficacy of formulated 2.5%, 5% and 10% Plumeria obtusa sprays in in-vivo wound healing models. The spray was prepared in three formulations F1, F2 and F3 containing 2.5% (50 mg/kg BW), 5% (100 mg/kg BW) and 10% (200 mg/kg BW) extract of P. obtusa leaves respectively. The wound healing activity was studied by invivo method. In excision model the % wound closure rate on epidermal skin of white Wistar albino rats was studied and was compared with standard Hansaplast Wound spray. The tensile strength or skin breaking strength was measured on the 10th post wounding day in anesthetized experimental rats in incision model. The results obtained by 3 formulations F1, F2 and F3 were studied and compared with the control group. The formula F3 shows complete wound healing on day 17, while the F1 and F2 showed complete healing on day 21 and 19 respectively in excision wound model. In incision wound model the tensile strength was found to be high about 404 gm by application of formula F3 spray. Formula F1 and F2 showed 354 gm and 385 gm tensile strength respectively. The conclusion is ethanolic extract of the leaves of plant P. obtusa showed promising wound healing activity as studied by the invivo models. The study shows that Formula F3 with 10% (200 mg/kg BW) P. obtusa extract spray shows better and efficient wound healing activity than formula F1 with 2.5% (50 mg/kg BW) and formula F2 with 5% (100 mg/kg BW) extract spray.