Abstract
In the present study, hexane, ethyl acetate and methanol fraction of Amomum nilgiricum leaf was evaluated for antidiabetic efficacy through in vitro ?-amylase and ?-glucosidase assays, DPPH and H2O2 scavenging activities, followed by estimation of total phenol, total flavonoids and gas chromatography-mass spectrometry analysis. In the present study, a significant amounts of total phenolics (79.92±1.58 mg/g) and flavonoids (21.74± 0.89 mg/g) were showed from Ethyl acetae faction. Ethyl acetate fraction showed maximum inhibition of DPPH radicals (82.31±2.33%) with IC50 value of 52 µg/ml and H2O2 scavenging activity (97.62±2.89%) with IC50 value of 78.57 µg/ml concentrations. The ethyl acetate fraction was revealed maximum ?-amylase inhibition (77.23± 3.21%) with IC50 value 76.53 µg/ml. The ethyl acetate fraction recorded maximum ?-glucosidase inhibition (85.36±2.58%) with IC50 value 79.54 µg/ml. Ethyl acetate fraction exhibited maximum inhibitory activity of glucose movement into outer solution across dialysis membrane at 250 µg/ml as compared to the control. The ethyl acetate fraction revealed maximum insulin secretory activity (130.5±3.66%) in RIN-m5F cells. Methanol fraction recorded maximum glucose uptake percent in yeast cells (67.08±1.68%) when compared to standard metronidazole (68.06±0.73%). The GC-MS analysis of ethyl acetate fraction was recorded the presence of six phytochemical constituents. This study scientifically validates the antidiabetic activity of A. nilgiricum. Hence, in view of its comparative hypoglycemic strength, it can work as a valuable healing agent in treating diabetes.
Highlights
Diabetes is an utmost widespread and prolonged endocrine disease disturbing the metabolism of carbohydrate, lipid and protein [1]
Hexane, ethyl acetate and methanol fraction of Amomum nilgiricum leaf was evaluated for antidiabetic effi cacy, scavenging activities, followed by estimation of total phenol, total flavonoids
To assess the efficacy of A. nilgiricum extract as antidiabetic agents, we examined the influence on the ability of glucose transport across yeast cell membrane, glucose diffusion and insulin secretion
Summary
Diabetes is an utmost widespread and prolonged endocrine disease disturbing the metabolism of carbohydrate, lipid and protein [1]. In India, diabetes population is estimated to be 61.3 million, and it may increase up to 101.2 million until 2030 [5]. This large number which accounts for nearly 20% of the global patients, has deemed India as the world’s capital of diabetes [6, 7]. It is evident that diabetes influences modifications in the actions of the antioxidant enzymes in numerous tissues. This oxidative stress further involved in the improvement and advancement of diabetes related problems. Antioxidants show significant functions in free radicals scavenge with defending cells by
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