Evaluation of in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius and Trianthema decandra

Abstract

Glinus oppositifolius and Trianthema decandra belonging to the Ficoidaceae family were commonly used by tribal peoples for the treatment of liver disorders and cancer. The preliminary phytochemical screening of those plants showed the presence of flavonoids, terpenoids, tannins and saponins. The aim of this study was to evaluate the in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius (MEGO) and Trianthema decandra (METD). The antioxidative capacities of MEGO and METD were determined by the following four complementary assay; DPPH radical scavenging assay, superoxide anion generation by xanthine-xanthine Oxidase assay, hydroxyl radical scavenging assay and <TEX>$Fe^{2+}$</TEX>-ascorbate induced by lipid peroxidation assay. The <TEX>$IC_{50}$</TEX> values of the both extracts were calculated from the inhibition curve. The <TEX>$IC_{50}$</TEX> MEGO and METD in DPPH, superoxide anion, hydroxyl radical scavenging and lipid peroxidation assay are 1.85, 7.31, 13.95, 22.82 and 2.21, 9.78, 14.87, 19.76 <TEX>${\mu}g/ml$</TEX> respectively. Both the extracts exhibited a significant antioxidant effects.