Abstract
:Ethnobotanical report on vegetables such as Raphanus sativus (Radish), Trichosanthes cucumerina (Snake gourd), Solanum melongena (Brinjal), Brassica oleracea var. capitata (Cabbage) and Brassica oleracea var. botrytis (Cauliflower) has revealed significant medicinal properties. In this background, an attempt was made to explore the antiglycation activity of the selected vegetables. The hot water extracts of Brassica oleracea var. botrytis and Solanum melongena has recorded high (85.45±1.96 %) and low (58.86 ± 0.26 %) antiglycation activity, respectively. An improved antiglycation activity (85.37 %, 1.45 fold increase) through central composite design (CCD) based response surface methodology (RSM) was observed in Solanum melongena. Two strong spots belong to flavonoid (yellow color) and phenolic acid (blue color) group of compounds, isolated from Brassica oleracea var. botrytis has recorded 84.35 ±1.25 % antiglycation activity. The IC values of DPPH (diphenyl dipicryl hydrazine) and FRAP (ferric ion reducing antioxidant power) assays adopted for 2D PTLC eluate of Brassica oleracea var. botrytis was found to be 269.37 µg/ ml (r2=0.9677 and p=0.0025) and 51.61 µg/ ml (r2=0.9307 and p=0.0079), respectively. The LC-MS analysis of 2D PTLC eluate of Brassica oleracea var. botrytis has revealed the presence of polyphenols such as chlorogenic acid, methyl apigenin, catechin and one unknown compound.
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