Evaluation of In Vitro Anti-Cancer Activity of Kukkutanakhi Guggula on Liver, Prostrate, Ovary and Renal Cancer

Bharat Rathi,Mujahid B Khan,Ninad Sathe

doi:10.47552/ijam.v11i3.1582

Abstract

The medicines prepared by using exudates of Commiphora mukul (Stocks) Hook. are described in Ayurveda under Guggula Kalpana which are among such valuable dosage forms. According to retrospective literary review, the combination of Kukkutnaki (Aspidium cicutarium Sw.) and purified Guggula(Commiphora mukul (Stocks) Hook.) was first mentioned in the book Chikitsa pradeep named as Kukkutnaki Guggula. Since last 3 decades, it was documented as an herbal drug which is used for cysts, goiter, tumors, tonsillitis, abscess, mansvaha strotas ailments, which are burning issues worldwide. Due to its observed clinical efficacy in Arbuda (~Cancer), the current in-vitro anticancer study was conducted with an aim to check its anticancer effect on human hepatoma cell line-HEPG2 of Liver; PC-3 and DU145 cancer cell lines of Prostate; Ovcar-3, A2780, SK-OV-3, PA-1 cancer cell lines of Ovary and ACHN renal cancer cell line of Kidney. The current in vitro study was conducted at ACTREC, Kharghar, Navi Mumbai. The selected cancer cell lines were procured from ATCC, USA and NCCS Pune. The Sulforhodamine B (SRB) Assay protocol was followed to observe the activity of the study drug. The positive control was Adriamycin in the study. The growth curve graphs were plotted and LC50, GI50, TGI values were calculated. Kukkutnakhi Guggula was found safe for oral administration, non- toxic at cellular level (LC50 values were > 160) and have moderate activity on HEPG2, Ovcar-3, DU145, ACHN cancer cell lines and had shown negligible activity on A2780, SK-OV-3, PA-1 and PC-3 cell lines. This work provides scope to study its effect on targeted cancers, specific in vivo scientific studies and human clinical trials for further researchers.

Highlights

Introduction were utilized by AyurvedicAcharyas while documenting Materia medica of Ayurveda.[3,4]Kukkutnakhi is an herb grown around the monsoon and rainy seasons and found along the foot hills of Sahyadri region. [5,6] It is botanically identified as Aspidium cicutarium Sw. of familyDrypteridaceae.( 7,8) Initially it is mentioned in the text “Gharguti Aushadhe” and it is called as Kombadnakhi, Bichava, Nirvishi and Waghchavdi.(9,10) The rhizomes of this herb being used as a folklore remedy for the treatment of Shotha, Shula, Arbuda etc. successfully.[11]
Kukkutnakhi Guggula was found safe for oral administration, non- toxic at cellular level (LC50 values were > 160) and have moderate activity on HEPG2, Ovcar-3, DU145, ACHN cancer cell lines and had shown negligible activity on A2780, SKOV-3, PA-1 and PC-3 cell lines
Guggula kalpana i.e medicines prepared by using exudates of Commiphora mukul has its own importance in the arena of ayurvedic therapeutics.[12]. Kukkutnaki Guggula was selected as a study drug according to retrospective literary review from various texts and scientific magazines and it is indicated in cysts, tumors, tonsillitis, abscess, elephantiasis etc.[13]. It was first mentioned in the text

Summary

Introduction

Introduction were utilized by AyurvedicAcharyas while documenting Materia medica of Ayurveda.[3,4]Kukkutnakhi is an herb grown around the monsoon and rainy seasons and found along the foot hills of Sahyadri region. [5,6] It is botanically identified as Aspidium cicutarium Sw. of familyDrypteridaceae.( 7,8) Initially it is mentioned in the text “Gharguti Aushadhe” and it is called as Kombadnakhi, Bichava, Nirvishi and Waghchavdi.(9,10) The rhizomes of this herb being used as a folklore remedy for the treatment of Shotha, Shula, Arbuda etc. successfully.[11]. Successfully.[11] Guggula kalpana i.e medicines prepared by using exudates of Commiphora mukul has its own importance in the arena of ayurvedic therapeutics.[12] Kukkutnaki Guggula was selected as a study drug according to retrospective literary review from various texts and scientific magazines and it is indicated in cysts, tumors, tonsillitis, abscess, elephantiasis etc.[13] It was first mentioned in the text “Chikitsa pradeep” for various Mansavaha strotas ailments.[14] in the text “Guggula kalpana”, The different kind of natural secondary metabolites produce by the Plant Kingdom which are being inspected for their anticancer activities leading to the development of new clinical drugs.[1] In human development and well-being the rise of Cancer worldwide is a major impediment in current era. Mujahid B Khan et al, Evaluation of In Vitro Anti-Cancer Activity of Kukkutanakhi Guggula its method preparation and proportion of the ingredients is mentioned.[15]. Current in-vitro anticancer study was conducted by following SRB assay with an aim to check anticancer effect of Kukkutnaki Guggula on human hepatoma cell line-HEPG2 of Liver; PC-3 and DU145 cancer cell lines of Prostate; Ovcar-3, A2780, SK-OV-3, PA-1 cancer cell lines of Ovary and ACHN renal cancer cell line of Kidney

Methods

Discussion

Conclusion