Evaluation of impairment of DNA integrity in marine gastropods ( Cronia contracta) as a biomarker of genotoxic contaminants in coastal water around Goa, West coast of India

Abstract

The measurement of the impairment of DNA in marine gastropod ( Cronia contracta) provides an insight into the genotoxic effects of contaminants on marine organisms along the Goa coast. The impact of genotoxic contaminants on Goan coastal environment was evaluated in terms of the loss of DNA integrity (expressed as the value of ‘ I’) in marine snails with respect to those from the reference site (Palolem) over a period from April 2004 to May 2005 using the technique of alkaline unwinding assay. The DNA integrity in marine snails was found to be significantly damaged at Dona Paula (58%), Vasco (73.5%), and Velsao (48.5%) during the monsoon period (July–August 2004). Similar trend in the loss of DNA integrity in marine gastropods was also detected during the post-monsoon (November–December 2004) and the pre-monsoon (April–May 2005) periods. The low integrities of DNA in marine gastropods at these sites can be attributed to exposure to genotoxic contaminants especially polycyclic aromatic hydrocarbons (PAHs) and toxic heavy metals (Pb, Cd, Cu, Fe, and Mn) prevalent in the marine environment as evident by their accumulation in the tissues of the marine snails inhabiting different sites along the Goa coast. The contaminant-induced DNA strand breaks in marine snails increased significantly at Dona Paula, Vasco, and Velsao clearly indicating the levels of contamination of the sites by genotoxic compounds in those regions. The genotoxic effects of contaminants were further substantiated by detection of the impairment (39%) of DNA integrity in marine snails in a field experiment in which the same species of marine snails ( C. contracta) collected from the reference site, Palolem, were deployed at Dona Paula and caged for 25 days for exposure to ambient marine pollutants. The impairment of DNA integrity in marine gastropods along the Goa coast can thus act as a biomarker for marine pollution monitoring of genotoxic contaminants.