Abstract
Purpose: The present study was conducted to evaluate the effect of tenoxicam on cellular and humoral immunity. Methods: Tenoxicam (2.5 - 10mg/kg) was administered at three different doses to three groups of mice and the cellular immune responses were studied using delayed hypersensitivity response (DTH) and cyclophosphamide-induced neutropenia while the humoral immune response was evaluated using hemagglutination test and mice mortality ratio. Normal saline and cyclophosphamide were used as negative and positive controls, respectively. Results: DTH assay resulted in a significant reduction in skin thickness (p 5 mg >2.5 mg> negative control group. A dose dependent reduction response was observed (p<0.05) in haemagglutination assay (HA). In mice lethality test mortality ratios of 2.5 mg, 5 mg, 10 mg tenoxicam were 60 %, 80% and 100 %, respectively, compared to 20 % and 100 % for normal saline group and cyclophosphamide, respectively Conclusion: The results suggest that tenoxicam suppresses both cellular and humoral immunity in mice. Keywords: Tenoxicam, Cellular immunity, Humoral immunity
Highlights
Infiltration of blood leucocytes to an infected area in the body triggers inflammatory response
Mice of group B showed the maximum change in skin thickness in comparison with the mice treated with tenoxicam
In cyclophosphamide induced neutropenia assay, a dose dependent reduction response was observed in tenoxicam treated groups
Summary
Infiltration of blood leucocytes to an infected area in the body triggers inflammatory response. Treatment groups B, C and D were administered with tenoxicam (2.5 mg, 5mg and 10mg respectively) intraperitoneally daily for total of 13 days while the mice for the control group (group A) were given saline solution for the same duration. Each of the mouse in groups A and B was administered normal saline and cyclophoshamide (150 mg/kg) i.p. for the same duration (28 days) while all the mice in treatment groups and control groups were injected intraperitoneally with 0.5 × 109 sheep’s red blood cells/ mouse i.p in phosphate buffer saline on 14th and 21st day of the test. All the mice in the tenoxicam treated groups (C, D and E) were received daily doses of 2.5 mg, 5mg and 10mg of tenoxicam intraperitoneally for 21 days while those in groups A and B were administered with normal saline and cyclophoshamide (150 mg/kg) i.p. respectively. Statistical Package for Social Sciences (SPSS for Windows version 16, SPSS Inc, and Chicago, IL, USA) was used
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