Abstract
Abstract Background: Two enzyme immunoassays, ELISPOT and sandwich ELISA, were compared in order to evaluate the production of interferon gamma (IFN-γ) in peripheral blood mononuclear cell (PBMC) cultures from
Highlights
Warble flies (Hypodermabovis, L. and Hypoderma-lineatum, De Villers) cause myiasis in cattle from the Northern Hemisphere, leading to a serious negative impact on animal productivity and welfare
Spontaneous secretion was observed in mitogen unstimulated cultures, the number of IFN-γ-secreting cells (SC) was higher in non-sensitized peripheral blood mononuclear cell (PBMC)
The stimulation with the mitogen phytohemaglutinin A (PHA) increased the number of IFN-γ-SC in both sensitized and non-sensitized PBMC cultures, with a more pronounced effect in the former
Summary
Warble flies (Hypodermabovis, L. and Hypoderma-lineatum, De Villers) cause myiasis in cattle from the Northern Hemisphere, leading to a serious negative impact on animal productivity and welfare. Newly-hatched larvae penetrate the skin aided by the secretion of enzymes from the parasites’ midgut (hypodermins A, B and C) They migrate through fascial planes of muscles and connective tissue to the oesophageal submucosa, where they over winter before migrating to, and eventually completing their development in, the subdermal tissues of the back [1]. It is well known that young cattle are the most susceptible to Hypoderma infestation, since old cattle develop acquired resistance after repeated exposures to Hypoderma larval antigens [3]. This resistance has been recognized as an important factor in controlling larval populations, depending on both the host age and the number of larvae invading the host [4]. ELISPOT and sandwich ELISA, were compared in order to evaluate the production of interferon gamma (IFN-γ) in peripheral blood mononuclear cell (PBMC) cultures from Hypoderma (Diptera: Oestridae) infested cattle
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