Evaluation of hypoglycemic effect of compound(s) from petroleum ether fraction of ethanol extract of Mangifera indica red leaves

Abstract

– The petroleum ether fraction of ethanol extract of Mangifera indica red leaves decreased fasting blood glucose (FBG) level of approx. 90% in alloxan induced diabetes rats (AIDRs). However, the specific compound(s) has not been clarified. The present study has been designed to separate hypoglycemic compound(s) using preparative thin layer chromatography (PTLC) from petroleum ether fraction of ethanol extract of Mangifera indica red leaves. Development of TLC demonstrated the presence of distinct three layers having the Rf values 0.86, 0.56 and 0.28 on solvent system, chloroform: nhexane (80:20). Compounds with Rf values 0.86 and 0.56 reduced fasting blood glucose level by 92 and 96%, respectively in AIDRs. However, the compound with Rf value 0.28 did not have any significant antihyperglycemic effect. It was found from the experiment that the compound with Rf value 0.56 had predominant hypoglycemic effect than the other. Therefore, it can be a potential candidate for screening the lead for newer antidiabetic drugs that can offer a natural key to manage diabetes for the future.