Abstract

Milk and dairy products represent potential nutritive environment for the growth of some pathogen bacteria such as Salmonella spp., Escherichia coli, Staphylococcus aureus . Our study aimed to assess the incidence of pathogen bacteria through classical and molecular methods in order to perform a risk analysis in Transylvanian production area. The material was represented by 160 ripened dairy products from the following categories: soft, medium-hard and hard. We have isolated E.coli in 121 samples (75.62%), which represent a concerning number. All the samples were confirmed by PCR method targeting the stress protein A gene sequence. We did not find positive samples for Salmonella spp. and Staphylococcus aureus. At the risk analysis performed we found a medium level of probability and gravity for consumer contamination with pathogenic E.coli by consumption of dairy products. We concluded that further studies are needed in order to assess the toxicity of the E.coli strains isolated from the dairy products investigated so as to accurately assess the risk for contamination. We recommend a more rigorous hygiene during the milking and manufacturing of dairy products, especially within the traditional systems.

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