Abstract
Two hydride-based stationary phases (cholesterol and bidentate C18) are evaluated for use in chromatographic analyses with mass spectroscopic detection. The stability of the bonded moieties and the background signal in the effluent from these columns are investigated. Separations of steroids and catecholamines are used to characterize these separation materials. Peak symmetries and efficiencies can provide additional insights into the effectiveness of the two phases. In one case a small column configuration directly connected to the mass spectrometer inlet is also tested.
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