Evaluation of high-grade sodium bentonite on performance and immune status of broilers, fed ochratoxin and aflatoxin

Abstract

An experimental trial with 2×2×2 factorial design was conducted to assess the individual and combined effects of mycotoxins including ochratoxin A (OTA) (0 and 1.0 mg/kg), aflatoxin B1 (AFB1) (0 and 0.5 mg/kg) and high-grade sodium bentonite (HGSB) (0 and 1.0%) in broilers. Significant depression of body weight, feed consumption, serum protein and serum albumin were noticed due to OTA, AFB1 and OTA+AFB1. The relative weights of pancreas, spleen and bursa were not altered significantly either by inclusion of OTA, AFB1, OTA+AFB1 or HGSB. Increases in the relative weights of liver and gizzard were observed in OTA, AFB1 and OTA+AFB1. Decreases in serum protein levels and increases in gamma glutamyl transferase were noticed in the OTA+AFB1 group. Also decreases in serum protein levels for alanine amino transferase and thymus were observed. The antibody titres against Newcastle Disease and Infectious Bursal Disease were significantly (P<0.05) decreased in all the treatment groups compared to control. HGSB reduced the toxicity of aflatoxin and marginally ameliorated the effect of OTA+AFB1 on some parameters.