Evaluation of Hesperidin Protective Effect on Lipopolysaccharide-Induced Inflammation and lipid Peroxidation in BALB/C Mail Mice

Abstract

Hesperidin is a flavonoid found mainly in citric, has been shown to reduce inflammation. In the present study, we have tested the ability of hesperidin to inhibit lipopolysaccharide (LPS)-induced cytotoxicity and pro-inflammatory mediator production in vivo. Mice (8-9 week age) was divided into six groups (10 in each) and treated for 5 days as the following: control group received (0.02 % DMSO), the second and third group were injected intraperitoneally with hesperidin only at dose of 5, 10 mg/kg b.w respectively. While the fourth and fifth groups were co-treatment with 5, 10 mg/kg b.w hesperidin for two hours followed by LPS for 90 min, and the sixth group was injected with LPS only and represented as a positive control. Mice received LPS challenge exhibited highly significant increase in IL-6 and IL1-s cytokine levels along with significant MDA level increased compared to control group. On the contrary, mice that had received hesperidin showed a significant improvement by decreasing the cytokines of interest level and MDA before and after LPS challenge. Pre-treatment with quercetin showed protection effective in acute injury and suppression of inflammation induced by LPS, with suggestion of its potentially therapeutic role. Further study of the effects of other herbal constituents are critical to evaluating efficacy and elucidating their mechanisms of action.