Abstract
We introduced human CYP1A1 and CYP2B6 cDNAs into a japonica rice (Oryza sativa cv. Nipponbare), to establish two different transgenic rice lines, because both CYP1A1 and CYP2B6 metabolize several herbicides having different chemical structures and modes of biological action. These transgenic rice plants showed broad cross-tolerance toward various herbicides and metabolized them. The introduced human P450 protein essentially just enhanced the natural metabolism of herbicides in the transgenic rice plants. These transgenic rice plants should be important not only for developing herbicide-tolerant lines of rice, but also for decreasing herbicide residues in rice. In addition, transgenic rice plants should prove useful in implementing effective, inexpensive methods of decreasing various chemicals that are widespread in agricultural environments.
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