Evaluation of Hepatoprotective Activity with different Fractions of Gardenia gummifera Linn. on Paracetamol Induced Liver Damage in Rats

Pradeep Kumar Sabbani,Ravi Chander Thatipelli

doi:10.4172/2157-7609.1000199

Abstract

The hepatoprotective and antioxidant activity of methanolic extract of whole plant of Gardenia Gummifera (GGME) was evaluated against paracetamol induced liver damage in rats. And the GGME fractionated based on polarity of solvents with toluene, ethanol, 2-butanone, n-butanol and petroleum ether. The substantially elevated serum enzymatic levels of Aspartate Aminotransferase (AST), Alanine Transaminase (ALT), Alkaline Phosphate (ALP) and total bilirubin were restored towards normalization significantly by the GGME in a dose dependent manner in paracetamol induced liver damage. The biochemical observations were supplemented with histopathological examination liver sections high protection against paracetamol induced hepatoxicity. Further investigation continued with GGME fractions, 2-Butanone Fraction (BTF) and nbutanol Fraction (BAF) substantially elevated serum enzymatic levels of AST, ALT, ALP and Total Bilirubin (TB) were restored towards normalization significantly The significant values showed with n-butanol fraction on pentobarbitone induced sleeping time in mice and the liver weight of paracetamol induced liver damaged in rats. Meanwhile, in vitro antioxidant activities such as DPPH scavenging assay was also screened which were also found significantly positive in a dose dependent manner. The results of this study strongly indicate that GGME and n-butanol fraction results potent hepatoprotective activity against paracetamol induced liver damage in experimental animals.

Highlights

In one of our field survey we found an herb with beautiful tender fruits by name Gardenia gummifera (Rubiaceae)
Acute toxicity study Mild adverse effects and no mortality rate of the animals were observed during the period of 48 hrs study up to the dose 2000 mg/kg b.w. of the Gardenia Gummifera (GGME)
The GGME fractions TLF, Butanone Fraction (BTF) and Butanone (BNF) and nButyl Alcohol (BAF) exhibited Mild adverse effects and no mortality upto a dose of 1000 mg/kg b.w

Summary

Introduction

In one of our field survey we found an herb with beautiful tender fruits by name Gardenia gummifera (Rubiaceae). In Indian System of Medicine, the gum Dikamali is one of the important drugs, upon literature survey it was found that research has been carried out on the antibacterial [2], sedative, analgesic, antianxiety [3], hypocholesterolemic [4] Anticonvulsant [5] and anticancer activity [6] And flavones including gardenin, flavonoids, tannins, elagic acid, anthraquinones, phenolic acids, Dikamaliartane-A [6] and Triterpenoids [7] from stem bark of Gardenia gummifera Oleanoic aldehyde, sitosterol, D-mannitol, erythrodiol and 19 hydroxyerythrodiol have been reported from the plant Except these studies, so far no other biological and phytochemical investigations have been reported on the whole plant. The toxicity of the extract was assed in animal models

Methods

Results

Discussion

Conclusion