Evaluation of Hepatoprotective Activity of Aqueous Extract of Andrographis paniculata in Wistar Rats

Abstract

International Journal of Pharmacology and Clinical Sciences,2016,5,4,113-11.DOI:10.5530/ijpcs.5.4.4Published:December 2016Type:Research ArticleAuthors:Naveen Alasyam, Venkatanarayana Narapogu, Premendran John, Shaik Ubedulla, and Naveen Pokala Author(s) affiliations:Naveen Alasyam1*, Venkatanarayana Narapogu2, Premendran John3, Shaik Ubedulla4, Naveen Pokala2 1Ph.D Scholar, Department of Pharmacology, Mamata Medical College, Khammam. Telangana, INDIA. 2Assistant Professor, Department of Pharmacology, Mamata Medical College, Khammam, Telangana. INDIA. 3Professor, Department of Pharmacology, Mamata Medical College, Khammam. Telangana, INDIA. 4Associate Professor, Department of Pharmacology, Mamata Medical College, Khammam, Telangana, INDIA. Abstract:Background: The objective of the current study was to evaluate the hepatoprotective activity of the aqueous extract of Andrographis paniculata in wistar rats. Materials and Methods: The animals were divided into six groups with six rats in each group. First group was taken as control, and received 0.9% normal saline 10 ml/kg body weight orally. Second group was taken as CCl4 control group and treated with normal saline (10 ml/ kg, p.o.) and CCl4: olive oil (1:1, 2 ml/ kg, i.p. on 2, 5 and 8th day) daily for 20 day. The third group rats was treated with Liv 52- 100 mg/kg orally and CCl4: olive oil (1:1, 2 ml/ kg, i.p. on 2, 5 and 8th day) for 20 days .The fourth and fifth groups were taken as test groups and they received crude extract of Andrographis paniculata orally once daily for 20 days at the dosage of 250 and 500 mg/kg respectively. CCl4: olive oil was given i.p on 2, 5 and 8th day. On the 21st day, blood samples were collected by cardiac puncture method. The blood samples were used to estimate biochemical parameters like serum SGOT,SGPT, ALT, bilirubin and cholisterol and the liver was preserved for histopathological examination. Results: It was found that the Andrographis paniculata exhibited moderate protective effect by lowering the serum levels of SGOT,SGPT,ALT, bilirubin and cholisterol (P<0.001). Histology of liver section of animal treated with the extracts showed the hepatic cell regeneration. Conclusion: Andrographis paniculata has dose dependant hepatoprotective activity. Keywords:Andrographis paniculata, Carbon Tetrachloride, Hepatoprotective Activity, Herbals, Liver diseaseView:PDF (597.09 KB) PDFClick here to download the PDF file. Images [Normal control] shows liver parenchyma with intact architecture