Evaluation of Hematological Changes in Tre-En-En and Vitamin C Dietary Supplementation to Wistar Rats

Abstract

In recent times, hematological health indictors have been the focus of most debates, even though several researches on different animal models have been conducted relative to it. In this study, we evaluated the effects of dietary supplementation with Tre-en-en concentrates on full blood count [packed cell volume (PCV), total white blood cell count (TWBCC), total red blood cell (TRBCC) count and total Platelet count (TPC)]. Twenty (20) acclimatized (for two weeks) male rats between 150-200 g were procured and grouped into four of five rats each. Group I animals were fed with standard rat chow and water ad-libitum (normal control), while group II rats received 5µm each of Tre-en-en grain concentrate daily for five weeks. Whereas, groups III and IV respectively received vitamin C and Tre-en-en (5µm) + vitamin C (Co-administration) daily for five weeks. Following period of administration, animals were euthanized under chloroform anaesthesia and blood sample obtained for haematological analysis. Full blood count was then performed and comparisons made between groups to ascertain the changes in hematological health markers. Results from one-way analysis of variance (ANOVA) showed a statistically significant increase (p < 0.05) in Tre-en-en and Tre-en-en + Vit. C. treated groups (II and IV) compared to control group. Also, Tre-en-en Supplement fed groups (II and IV) showed a statistically significant increase in body weights than control and vit. C. groups (I and III). There was also a statistically insignificant increase (p < 0.05) in the PCV of Tre-en-en and Vit. C treated groups (II and III) compared to control, while TWBCC increased insignificantly across groups; whereas TRBCC increased significantly as compared to control. More corroborative works relative to this is recommended to validate the result of this study.