Abstract
In order to study the variation of four thyme species for aerial parts biomass, essential oil content and composition, this study was carried out in Zagheh, Rangeland, Khoramabad, Iran. Seeds of four species of Thymus pubescens, T. daenensis, T. lacnifolius and T. kotschyanus were collected from their natural habitat in Lourestan province, Iran. Seeds sown in Jiffy pots in glasshouse in Septmber 2016 and the seedlings were transferred to field in October 2016 using Randomized Complete Block Design (RCBD) with 3 replications in Khoramabad, Iran. Each unit of experiment consists of 40 plants in two rows with 50 cm distance between rows and 50 cm between plants within rows. Data were collected for aerial parts biomass, morphological, traits and essential oil percentages over two years 2017-2018. The essential oils of all samples were obtained by hydro-distillation, in flowering stage and the essential oil components were identified using gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS). Considerable variation was found between species for aerial parts fresh and dry biomass, plant height, root length and essential oil content. The mean values of aerial parts biomass, crown diameter and essential oil in T. daenensis were higher than other species. For the root length the higher value was obtained for T. pubescens. The lower and higher essential oil content with average values of 1.53% and 1.95% were obtained in T. pubescens and T. lancifolius, respectively. The results obtained in our study indicated that the major components in the essential oil were carvacrol (5.8, 18.5, 42.4 and 33.5%) and thymol (78.9, 51.9, 36.19 and 42.7%) for T. daenensis, T. kotschyanus, T. lancifolius and T. pubescens, respectively.
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