Evaluation of glycine betaine as an inhibitor of dissolved dimethylsulfoniopropionate degradation in coastal waters

Abstract

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 128:121-131 (1995) - doi:10.3354/meps128121 Evaluation of glycine betaine as an inhibitor of dissolved dimethylsulfoniopropionate degradation in coastal waters Kiene RP, Gerard G Dimethylsulfoniopropionate (DMSP) is an organic sulfur compound which is produced by many marine phytoplankton and which is ubiquitous in the euphotic zone of the ocean. DMSP is degraded through complex interactions within the food web and studies of its dynamics may lead to greater understanding of microbial ecology and food web interactions. In this study we examined the degradation of dissolved DMSP [DMSP(d)] in coastal water samples and tested glycine betaine (GBT), a structural analog of DMSP, as a potential inhibitor of this important biogeochemical reaction. The addition of 1 to 50 uM GBT to water samples from the northern Gulf of Mexico strongly inhibited the consumption of 50 nM added DMSP(d). The production of dimethyl sulfide (DMS) from DMSP(d) was also inhibited by GBT, but was slightly less sensitive than overall DMSP degradation. The inhibitory effects of GBT were short-lived, lasting only 5 to 6 h, after which time net DMSP(d) consumption resumed. Several analogs of GBT were also found to be inhibitory to DMSP(d) degradation but unrelated compounds had no effects. Consistent with the inhibitory effects of GBT, we found that endogenous DMSP(d) concentrations increased at steady rates in response to GBT additions. These GBT-induced accumulation rates ranged from 4 to 28 nM d-1 in water samples collected over the course of a year and may represent the natural turnover rates of DMSP(d). We found no significant effects of GBT on particulate DMSP concentrations in natural water samples or in an axenic culture of the prasinophyte Tetraselmis subcordiformis. However, addition of 50 uM GBT to the phytoplankton culture caused an accumulation of DMSP(d) (equivalent to 2% of the particulate DMSP in the culture) for a period of 1 h with no change thereafter. GBT may be a useful inhibitor of DMSP(d) degradation (and DMS production) under some circumstances. However, the short-lived inhibitory effects of GBT and the potential for it to cause some direct release from the particulate DMSP pool may limit its application. Dimethylsulfide . Tertiary sulfonium . Quaternary ammonium . Climate . Biogeochemistry . Inhibition . Demethylation . Lyase Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 128. Publication date: November 23, 1995 Print ISSN:0171-8630; Online ISSN:1616-1599 Copyright © 1995 Inter-Research.