Evaluation of Global Gene Expression During Cold Shock in the Human Pathogen Vibrio vulnificus

Abstract

Vibrio vulnificus can adapt to cold temperatures by changing the expression profiles of certain genes and their resulting proteins. In this study, the complete V. vulnificus transcriptome was analyzed under cold shock by looking at gene expression changes occurring during the shift from 35°C to 4°C. A DNA microarray-based global transcript profiling of V. vulnificus showed that 165 genes out of 4,488 altered their expression profiles by more than twofold. From 35°C to 10°C, an overall gene repression was observed while changes occurring below 10°C mainly resulted in upregulation. The highest induction observed occurred in two of the five categorized cold-shock genes, cspA and cspB, which showed a complementary expression pattern during cold shock suggesting a homologous role. Other genes showing a significant fold increase included ribosomal genes, protein folding regulators, and membrane genes. Repressions were observed in all orthologous groups. Genes with top fold changes in repression include those coding for catalytic enzymes responsible for non temperature-related stress regulation. These included antioxidants, sugar uptake, and amino acid scavengers. V. vulnificus maintained a high level of cspA and cspB transcripts during the entire experiment suggesting that these class I cold-shock genes are required beyond the initial phase of the acclimation period.