Abstract
Streptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers (9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteers.
Highlights
Dental caries is a multifactorial infectious disease, related to biofilm accumulation on dental surface[16] and frequent consumption of fermentable carbohydrates[2]
Different genotypes of S. mutans have been found in saliva, and dental biofilm and AP-polymerase chain reaction (PCR) technique has been widely used to discriminate this genotypic diversity[1,6,7,9,11,12,18,23,26]
The aim of the present study was to evaluate the degree of agreement of genotypic identification between APPCR reactions performed with distinct arbitrary primers (OPA 02, OPA 03, OPA 05, OPA 13 and OPA 18) of Streptococcus mutans isolated from saliva of adult volunteers
Summary
Dental caries is a multifactorial infectious disease, related to biofilm accumulation on dental surface[16] and frequent consumption of fermentable carbohydrates[2]. Different genotypes of S. mutans have been found in saliva, and dental biofilm and AP-PCR technique has been widely used to discriminate this genotypic diversity[1,6,7,9,11,12,18,23,26]. This technique has a discriminatory potential comparable to other techniques for genotypic identification of S. mutans[13,14,28]. Et al.[29] (2000), investigating genotypic diversity among mutans streptococci, verified that
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