Abstract
Vitis amurensis ‘Cheongsan’ is a plant with high economic value in both medical and agricultural applications. However, its utilization has been restricted owing to difficulties encountered when applying traditional mass propagation methods, requiring instead application of in vitro propagation methods for their mass scale production. Hence, this study was conducted to find the optimal plant growth regulators for shoot multiplication and root induction during in vitro propagation. Among the three cytokinins used at multiple concentrations for culture initiation and shoot multiplication, the most positive response was found with MS medium containing 5.0 μM 6-benzyladenine (BA), compared to more modest responses from other types of cytokinin, such as kinetin (KIN) and thidiazuron (TDZ). For root induction, medium supplemented with α-naphthaleneacetic acid (NAA) produced a callus and inhibited shoot growth in explants, whereas indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) did not create any significant problems, but did display differences in root induction efficiencies. Generally, root induction responses with IBA were better that those with IAA. The maximum rooting rates were observed without callus formation and no shoot growth inhibition from explants grown on media supplemented with 0.67 μM IBA. Further, inter-simple sequence repeat (ISSR) analyses revealed that micropropagated plantlets generated in medium supplemented with 5.0 μM BA and 0.67 μM IBA did not lead to genetic variation. Therefore, the application of the in vitro propagation method developed in this study could be used on a commercial scale and will offer opportunities to strengthen the industrial use of V. amurensis ‘Cheongsan’.
Highlights
Vitis amurensis belongs to the family Vitaceae
Nodal segments grown in media containing cytokinins were regenerated with callus formation after 10 days of culture and the generation of new axillary buds was frequently observed after 3 weeks of culture
Further investigation is needed to provide a clear explanation of these observations, our results indicate that application of indole-3-butyric acid (IBA) might be more effective for the in vitro propagation of V. amurensis ‘Cheongsan’ in terms of stability, persistence, and toxicity protection in media when utilizing our protocol
Summary
Vitis amurensis belongs to the family Vitaceae It has been regarded as an important medical crop and has long been used in various ethno-medical systems because it contains an abundance of resveratrol and anthocyanin, which have excellent anti-cancer, anti-oxidation, and antiinflammatory effects (Jang et al, 2007; Bak et al, 2016; Chen et al, 2018). It has received significant attention as a very useful breeding material, improving resistance to disease and the freezing tolerance of existing commercial grape varieties (Fennell, 2004; Chai et al, 2019; Fu et al, 2019).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
