Abstract
The aim of this study was clarifying the relation between genetic diversity of edible honeysuckle (Lonicera kamtschatica) and the major group of biologically active substances as total polyphenols content (TPC) including antioxidant activity (AO). Fruits of edible honeysuckle becomes more and more popular, especially in Europe. The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed.The biological material including 14 cultivars of the edible honeysuckle (´Zoluška´, ´Amfora´, ´Pruhonický 44´, ´Vasilijevsky´, ´Moskovskaja´, ´Vojtek´, ´Sinoglaska´, ´Altaj´, ´Lipnická´, ´Kamčadalka´, ´Sinaja Ptica´, ´Fialka´, ´Modrý Triumf´, and ´Leningradský velikán´) originated from Czech republic (Žabcice near Brno). The content of TPC and AO were determined by location and its soil-climatic conditions and these environmental circumstances determines the RAPD profiles of analysed honeysuckle acessions, too. DPPH method was used to analyze AO and Folin-Ciocalteu method was used to determine TPC. The results of experiment showed that the highest value of AO was determined at the cultivars ´Zoluška´ (81.04 mg.L-1) and the lowest was measured in ´Kamčadalka´ (54.122 mg.L-1). On the contrary, the highest content of TPC was determined at the cultivar ´Kamčadalka´ (51.09 mg.L-1) and the lowest value was measured at the cultivar ´Pruhonický 44´ (21.65 mg.L-1). Phylogenetic trees were similar in genetic distance. The content of TPC and AO were not statistically significant in relation to cultivar. The analyzed cultivars of the edible honeysuckle were separated in 4 clusters according to used primers. In both gel images, the amplicon size ranged from 100 to 1,500 bp. We found that genetic diversity was partially related to content of total polyphenolic substances and antioxidant activity. Based on phylogenetic trees we have stated that variety ´Lipnická´, ´Sinoglaska´, ´Altaj´, ´Leningradský velikán´, ´Modrý Triumf´, ´Sinaja Ptica´ and ´Kamčadalka´ were grouped in the similar cluster. The highest genetic distance was determined at the variety ´Vasilijevskaja´ and ´Amfora´. In the same way, there were variety ´Vojtek´, ´Fialka´ and ´Zoluška´.
Highlights
IntroductionDespite their valuable qualities have been less well-known fruits species in the territory of Slovakia
Fruits of edible honeysuckle, despite their valuable qualities have been less well-known fruits species in the territory of Slovakia
The aim of this study was clarifying the genetic diversity of selected cultivars of edible honeysuckle in relation to content of the predominant group of the biologically active ingredients sumed up as total polyphenols content (TPC) and antioxidant activity (AO) of fruits
Summary
Despite their valuable qualities have been less well-known fruits species in the territory of Slovakia. Edible honeysuckle come from the territory of the Russian Federation. From the point of view of the soil-climatic conditions of the locality, the al plants are not demanding Among their precious properties in terms of growing conditions are high freezing resistance as well as resistance to diseases and pests (the incidence of plant diseases affected by diseases and pests is very low). Their growing importance based on the early flowering period, which result in earlier planting of the plants, significantly reducing the length of the growing season (Matuškovič et al, 2003). The fruit of edible honeysuckle have been rich in phenolic acids, flavonoids (quercetin, rutin, anthocyanins) and ascorbic acid content too (Juríková et al, 2012)
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