Abstract
ABSTRACTFour populations of Piptoporus betulinus from Maoer, Changbai, Daxing'an and Xiaoxing'an Mountains were sampled. DNA was isolated and purified from fruiting bodies. Inter simple sequence repeat markers were first used to evaluate the genetic diversity and differentiation of P. betulinus in this study. The results showed that the strains from Xiaoxing'an Mountain had the highest diversity among the four populations, followed by the samples from Maoer and Daxing'an Mountains, whereas the Changbai Mountain samples harboured the lowest value. Cluster analysis and principal coordinates analysis indicated that all of the populations had the same type of geographical distribution (geographical affiliations). These findings will provide useful information for further research and utilization of wood-rotting fungi and will lay the foundation for development of genetic diversity in P. betulinus from various geographic origins.
Highlights
In nature, wood-rotting fungi play an important role in the cyclic utilization of timber
7 out of 100 inter simple sequence repeat (ISSR) primers with clear high-intensity and relatively high polymorphism bands were selected and used to amplify 15 P. betulinus strains from four populations
By analysing the number of alleles and polymorphic loci (PPL) using ISSR, we found that the polymorphic loci (PL) ratio in the four locations ranged from
Summary
Wood-rotting fungi play an important role in the cyclic utilization of timber. The optimal growth temperature of brown-rot fungi is 27–38 C [6]; P. betulinus is suitably grown in northern China with good distribution, such as in the Heilongjiang, Inner Mongolia and Liaoning Provinces. This brown-rot fungus is especially parasitic on Betula platyphylla [8]. Inter simple sequence repeat (ISSR) markers are co-dominant traits following Mendelian patterns This method has the advantages of random amplified polymorphic DNA and amplified fragment-length polymorphism. Foundation for further study and reasonable application of wood-degrading fungi
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